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EcoRV and XbaI digestion revealed one band of around 5000 bp. EcoRI digestion produced two bands of around 3600 and 1600 bp. After HindIII digestion 3 bands were visible of around 3000, 1500 and 400 bp. Sequence analysis later showed that HindIII digestion in fact produced four fragments of which the smallest, 378 and 364 bp, coincided on gel.
