Table 1. Transcription of the com T4SS is induced by DNA damage but recA expression is not.
Genotype tested | recA | comB8 | comB9 |
Wild-type plus cipro | −1.9 (−1.1–−3.1) | 3.5 (2.7–4.4) | 4.3 (3.5–5.2) |
ΔaddA | −1.7 (−1.1–−2.9) | 5.5 (4.3–7) | 6.6 (4.9–8.9) |
ΔrecA | ND | 1.2 (1.0–1.5) | 1.4 (1.0–1.8) |
ΔrecA plus cipro | ND | ND | 1.2 (1.0–1.3) |
comB4IE | ND | ND | 3.1 (2.7–3.6) |
ΔcomB10 ΔaddA | ND | 1.6 (1.3–2) | 2.1 (1.7–2.7) |
ΔcomB10 plus cipro | ND | ND | 1.1 (0.9–1.3) |
The fold change in transcription (range) for the indicated gene was measured for cells of the indicated genotype by real-time PCR using the comparative method. ND: not done.