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. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: Anal Bioanal Chem. 2010 Apr 28;397(8):3377–3385. doi: 10.1007/s00216-010-3728-2

Fig. 2.

Fig. 2

(A) Process flow of the contact printing method using the PAA sacrificial layer: 1. Spin PAA on glass slide, 2. Contact the micropallet array with the PAA film, 3. Remove the array leaving PAA printed on the top surface of each micropallet, 4. Silanize the PAA printed array, 5. Remove PAA, 6. Spin polystyrene on glass slide, 6. Contact the array with the polystyrene film, 7. Remove the array leaving polystyrene printed only on the top surface of the micropallets. (B) Image of the upper surface of a micropallet 10 min after contact printing with polystyrene as described in “A”. (C) Micrographs showing PAA coverage on the upper surfaces of individual micropallets under various conditions. (D) Histogram of PAA coverage area on the micropallet surface under varied conditions.