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Inter-dependence of observed enthalpic change (ΔHobs), entropic change (TΔSobs) and free energy change (ΔGobs) for the binding of ERE duplex to the wildtype DB domain of ERα in various buffers. (a) ΔHobs-TΔSobs plot. (b) ΔHobs-ΔGobs plot. (c) TΔSobs-ΔGobs plot. Note that the solid lines represent linear fits to the data in all plots. All error bars were calculated from 3–4 independent measurements and are given to one standard deviation.
