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. Author manuscript; available in PMC: 2011 Jul 27.
Published in final edited form as: Biochemistry. 2010 Jul 27;49(29):6177–6183. doi: 10.1021/bi100775t

Figure 3.

Figure 3

Western Blot analysis of endogenous PARP-1 binding to different DNA probes. (A) Binding of unactivated PARP-1 to cDDP-G*G* (lanes 1-3), cDDP-G*TG* (lanes 4-6), cDDP-ICL (lanes 7-9), and NoPt (lanes 10-12). (B) Binding of activated PARP-1 to cDDP-G*G* (lanes 1-3), cDDP-G*TG* (lanes 4-6), cDDP-ICL (lanes 7-9), and NoPt (lanes 10-12). A 45 μL quantity of CFE was used in all lanes. The samples were resolved on 8% SDS-PAGE and detected using a PARP-1 antibody.