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. Author manuscript; available in PMC: 2011 Jul 27.
Published in final edited form as: Biochemistry. 2010 Jul 27;49(29):6052–6063. doi: 10.1021/bi1006942

Table 1.

Stability parameters for the apoMb variants1

ApoMb Variant KNI0 KIU0 1/KNU0
WT 0.021 0.019 2500
H64F 0.0061 0.011 15000
T67P 0.67 0.029 51
V68T 0.13 0.017 450
H97D 0.013 0.061 1300
1

The GuHCl-induced unfolding measurements were carried out in 10 mM potassium phosphate pH 7, 20 °C. The KNI0 and KIU0 were obtained by fitting simultaneously both CD and Fluorescence signals for the unfolding of the apoMb variants to the 3-state unfolding mechanism using Equation 3 (see Supplementary Material, Table S1). The values of mNI and mIU were set to 2.35 and 1.36 kcal mol−1 M−1, respectively.