Table 1.

The physical properties of telodendrimers with different PEG chain length and the number of cholic acids and lipophilic molecules.

Telodendrimers	Mw (theo.)	Mwa (MS)	Mwb (NMR)	CMC (μM)c	Size (nm)d	Size with PTX (nm)d	PTXe (mg/mL)
PEG2k-CA4	3914	4105	4511	7.9	11.5	15	5.2
PEG3k-CA4	5250	5606	5251	12.5	15	141	5.6
PEG5k-CA4	6644	6313	6082	67	10	131	2.3
PEG2k-CA8	5986	5985	6127	1.3	302/6000	96/348/1863	N/D
PEG3k-CA8	7322	7624	8025	5.9	20	58	4.7
PEG5k-CA8	8716	8814	8805	5.3	21	61	7.3
PEG10k-CA16	8172	N/D	N/D	0.97	42.7/2163	102/602/2435	0.6
PEG5k-CF8f	8909	N/D	N/D	0.4	122/280/827	134/388/905	N/D
PEG5k-LA8g	8460	N/D	N/D	1.8	73/6000	67/450/872	0.8
PEG5k-HA8h	7620	N/D	N/D	1.5	55/322	97/5990	N/D

^[a]Obtained via MALDI-TOF MS analysis, a-cyano-hydroxyl-cinnamic acid as a matrix compound.

^[b]Obtained via ¹H NMR method. Given the molecular weights of the starting PEGs by MALDI-TOF MS (Figure S-1 in supporting information), the molecular weight was calculated based on the ratio of proton signals of the methyl groups on cholic acid to the proton signals of the PEG in the ¹H NMR spectra.

^[c]CMC was measured by fluorescence spectrometry using pyrene (2×10⁻⁶ M) as a probe;

^[d]Measured by dynamic light scattering particle sizer (Nanotrac®);

^[e]PTX loading, in the presence of 20 mg/mL of telodendrimers, were measured by HPLC after passing through an 0.45 μm filter. N/D means not detectable;

^[f]CF = cholesterol formate;

^[g]LA = lithocholic acid;

^[h]HA = heptadecanoic acid.