Table 2.
Gene | Na | Lcb | Sc | dN/dSd | −2ΔlM8 versus M8Ae | −2ΔlM1 versus M2e | −2ΔlM7 versus M8e | Parameter Estimates from M8 | Positively Selected Sitesf |
CENP-A | 14 | 140 | 1.1 | 0.40 | 18.1** | 18.1** | 19.4** | p1 = 0.13, dN/dS = 3.5 | 7S, 17S, 18P, 35A, 39Q, 41S, 42R, 45Q, 46G, 62I, 65L, 76V |
p0 = 0.87, β(19.2, 99) | |||||||||
CENP-B | 16 | 618 | 0.9 | 0.06 | 0.0 | 0.0 | 0.0 | NA | NA |
CENP-C | 13 | 950 | 1.1 | 0.75 | 24.6** | 24.4** | 24.8** | p1 = 0.07, dN/dS = 3.9 | 12G, 24R, 64R, 83P, 99F, 106A, 108N, 117H, 126S, 132D, 133S, 136I, 177S, 192M, 229D, 240S, 256R, 283A, 287P, 291C, 294D, 296T, 297K, 325G, 331T, 332I, 372T, 385Y, 391T, 395Y, 405K, 412R, 417I, 429P, 436V, 444I, 445H, 446T, 450T, 452D, 453E, 465H, 468M, 472C, 479P, 481V, 499R, 506N, 526R, 553H, 558R, 567S, 572R, 589Q, 594F, 613S, 614L, 633C, 650Q, 653P, 670N, 676H, 679S, 690N, 699N, 707H, 715Q, 769S, 771V, 777I, 778S, 787I, 791N, 834E, 841V, 891V |
p0 = 0.93 β (0.15, 0.078) | |||||||||
C2orf18g | 14 | 371 | 0.8 | 0.05 | 0.4 | 0.54 | 7.86* | p1 = 0.03, dN/dS = 1.4 | 32M, 73A, 167H, 169S, 264V, 350L |
p0 = 0.97, β (0.66, 18.64) | |||||||||
SPEF1g | 16 | 236 | 1.1 | 0.14 | 0.0 | 0.0 | 0.0 | NA | NA |
STAP1g | 13 | 299 | 0.69 | 0.27 | 0.0 | 0.0 | 0.2 | NA | NA |
Dpysl5g | 14 | 564 | 0.54 | 0.02 | 0.0 | 0.0 | 0.0 | NA | NA |
CDC25g | 13 | 588 | 0.91 | 0.14 | 0.2 | 0.0 | 1.8 | NA | NA |
Number of taxa.
Length of alignment in codons.
Tree length.
Ratio of nonsynonymous to synonymous nucleotide changes.
For model comparisons (see text), significance is indicated with one (P < 0.05) or two asterisks (P < 0.0001).
For positively selected residues, those in bold have posterior probabilities >0.90, underlined 0.70–0.89, and regular font 0.50–0.69.
Non-CENP genes listed here are included to evaluate potential regional selection. C2orf18 and DPYSL5 flank CENP-A; SPEF1 and CDC25B flank CENP-B; and STAP1 is the only other complete gene within the analyzed CENP-C–containing region (see supplementary fig. 1, Supplementary Material online).