Figure 5.

IQGAP1 is a scaffold protein that facilitates mTOR interaction with Akt. HEK-293 cells were transiently transfected with equal amounts of Myc-IQGAP1(WT), Myc-IQGAP1ΔMK24. After 36 h, cells were lysed. (A) Immunoprecipitated with anti-Myc antibody for IQGAP1, and subjected to Western blot with anti-Myc, anti-mTOR, or anti-Akt. Con indicates total lysates from non-transfected cells. (B) Immunoprecipitated with anti-mTOR antibody, and subjected to Western blot with anti-Myc, anti-mTOR, or anti-Akt. (C) Immunoprecipitated with anti-Akt antibody, and subjected to Western blot with anti-Myc, anti-mTOR, or anti-Akt.