Figure 8.

Dedifferentiation by 2DG occurs through β-catenin accumulation in rabbit articular chondrocytes. (A) Chondrocytes were untreated or treated with 5 mM 2DG for 24 h in the presence of 20 µM ER-stress inhibitor salubrinal. Expressions of type II collagen, SOX-9, β-catenin and GAPDH were determined by RT-PCR. GAPDH was used as a loading control. (B) Primary chondrocytes were untreated or treated with 5 mM 2DG for 24 h in the presence of 20 µM salubrinal, ER-stress inhibitor. Expressions of type II collagen, SOX-9, β-catenin, Grp94 and β-actin were determined by Western blot analysis. Data are presented as results of a typical experiment.