Table 1.
Inhibition of 7dF3 reporter activity (IC50) and growth inhibition (GI50) of hit compounds
| Compound | Reporter IC50 (μM) |
HT29 (APC mutant) GI50 (μM) |
HCT116 (oncogenic β- catenin, GI50 (μM) |
SW480 (APC mutant) GI50 (μM) |
SNU475 (Axin mutant) GI50 (μM) |
CCD841Co (Control) GI50 (μM) |
|---|---|---|---|---|---|---|
| CCT007812 | 6.2 | 11.0 | 11.1 | 46.7 | 32.0 | >>44 |
| CCT014939 | 20.5 | >100 | >100 | >100 | 28.5 | >>44 |
| CCT020435 | 14.1 | 10.0 | 12.5 | 9.1 | ND | >>44 |
| CCT028492 | 2.7 | 8.0 | 14.1 | 11.7 | ND | ND |
| CCT031374 | 6.1 | 11.5 | 13.9 | 13.2 | 9.6 | >>44 |
| CCT036098 | 21.5 | >100 | 31.9 | 27.9 | 17.3 | >>44 |
| CCT036477 | 4.6 | 17.9 | 17.7 | 33.3 | ND | >>100 |
| CCT038407 | 5.3 | 3.0 | 2.3 | 2.5 | 21.1 | >>44 |
| CCT070535 | 5.1 | 17.6 | 11.1 | 11.8 | 13.4 | >>44 |
NOTE: HT29 (42), SW480 (39), and HCT116 (40) are colon carcinoma cell lines. SNU475 is a hepatocellular carcinoma cell line (29). CCD841Co is a colonic epithelial cell line whose use as a control has been described previously (5). Abbreviation: ND, not done. >>No growth inhibition at the highest concentration tested (indicated).