Figure 1.

Dnmt2 is a multisubstrate tRNA methyltransferase. (A) Quantitative PCR analysis of Dnmt2 mRNA expression in Drosophila tissues. Expression was normalized to rp49 levels. (B) Identification of novel Dnmt2 substrate tRNAs. Deep bisulfite sequencing analysis of various tRNAs in wild-type (control) and Dnmt2 mutant (Dnmt2 mut.) flies. Numbers of sequence reads are indicated at the top of each panel. Diagrams show fractions of nondeaminated cytosines, thus revealing the pattern of m5C modification in individual tRNAs. Cytosines that were found to be methylated in a Dnmt2-dependent manner are C38 in tRNA^Asp-GTC, tRNA^Val-AAC, and tRNA^Gly-GCC (highlighted by bold numbers).