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. 2010 Aug 1;24(15):1590–1595. doi: 10.1101/gad.586710

Figure 4.

Figure 4.

Stress-induced cleavage of Dnmt2 substrate tRNAs. (A) Northern analysis of tRNAGly-GCC cleavage in S2 cells that were heat-shocked (1 h at 40°C) for the times (in minutes) indicated. 5.8S rRNA was used as a loading control. (B) Cleavage of tRNAGly-GCC in S2 cells that were treated with H2O2 (1 mM H2O2, 60 min) or sodium arsenite (0.5 mM SA, 90 min). (C) Cleavage of tRNAGly-GCC in wild-type (D2+/+) or Dnmt2 mutant (D2−/−) ovaries (resected 35 d after hatching) that were heat-shocked (+) in medium at 40°C. Controls (−) show results from parallel experiments with ovary incubation at 25°C. (D) Northern analysis of tRNAGly-GCC and tRNAAsp-GTC cleavage in S2 cells that allow inducible overexpression of Dnmt2 (iD2). Full-length tRNAs are marked by gray arrows, and tRNA fragments are marked by black arrowheads.