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Ver directly binds HOAP and Moi. (A) GST-Ver precipitates both HOAP-Flag (HOAP-F) and Moi-HA from S2 cell extracts. (B) GST-Ver precipitates HP1-Flag (HP1-F) from S2 cell extracts. (C) GST-HOAP, but not GST-HP1, binds bacterially purified 6His-Ver. (D) GST-Moi binds bacterially purified 6His-Ver. (E) The 6His-Verm4 mutant protein binds GST-HOAP and GST-Moi; both 6His-Ver and 6His-Verm4 bind GST-Ver. Input is 10%. (IB) Immunoblotting.
