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. 2010 Aug 1;24(15):1602–1607. doi: 10.1101/gad.1940210

Figure 3.

Figure 3.

Loss of Puma prevents stem cell apoptosis upon IR. (A) Cellularity of both femora and tibiae was assessed in mice of the indicated genotypes after exposure to a single dose of IR. (B–D) Cell counting and staining with cell surface marker-specific antibodies were used to identify and enumerate the different stem cell populations. Bars represent mean ± SEM of three to six animals per genotype and time point from three independent experiments. (*) LSK cell numbers were significantly different between wild-type or bim−/− versus puma−/− (P < 0.0002) or p53−/− mice (P < 0.0014) at all time points analyzed after IR; MPP numbers were different between wild-type or bim−/− versus puma−/− (P < 0.04) or p53−/− mice (P < 0.035) at days 1, 3, and 7 after IR; LT-HSC numbers were different between wild-type or bim−/− versus puma−/− (P < 0.038) or p53−/− mice (P < 0.003) at day 7 after IR. (§) In untreated mice, LT-HSC numbers were different between bim−/− and all other genotypes (P < 0.05).