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. 2010 Aug 1;24(15):1634–1644. doi: 10.1101/gad.1941310

Figure 5.

Figure 5.

Dose response study of ASO 10–27 in the lumbar spinal cord after a single embryonic ICV injection. Using a glass micropipette, 2 μL of ASO 10–27 was injected into a cerebral lateral ventricle of individual embryos at approximately E15 (Smn+/− or Smn−/−; SMN2+/+) at the indicated doses. (A) The effect of ASO 10–27 on SMN2 splicing was assayed at P7. Total RNA isolated from the lumbar spinal cord was analyzed by radioactive RT–PCR as in Figure 1. Three representative samples from four to five treated mice are shown. (B) Quantitation of the dose response radioactive RT–PCR data from lumbar spinal cords obtained at P7 or P30 (n = 4–5). For all of the doses, the difference between P7 and P30 was significant (P ≤ 0.02, two-tailed Student's t-test). (C) Quantitation of the effects of ASO 10–27 in different spinal cord regions in P7 neonates; two doses (5 and 10 μg) were chosen for comparison. An asterisk (*) indicates a significantly lower percentage of exon 7 inclusion in the lumbar spinal cord than in the cervical (P = 0.017, n = 4, two-tailed paired Student's t-test) and thoracic (P = 0.016, n = 4) regions.