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. 2010 Aug 1;24(15):1634–1644. doi: 10.1101/gad.1941310

Figure 6.

Figure 6.

Phenotypic rescue of type III SMA mice (Smn−/−; SMN2+/+) after a single embryonic ICV injection of ASO 10–27. (A) Tail lengths were measured from P7 to 12 wk old, at weekly intervals. E15 embryos were injected with saline vehicle (n = 10) or 10 μg (n = 17) (ASO-10) or 20 μg (n = 11) (ASO-20) of ASO 10–27 in a volume of 2 μL. Normal mice (Smn+/+ or Smn+/−) were used as positive controls (n = 10). (B) Radioactive RT–PCR of tail RNA samples obtained from mice treated with saline or 20 μg of ASO 10–27 at approximately E15. (C) Representative 2-mo-old mice to show ASO rescue of tail and ear necrosis. The two mice on the left have normal tails and ears (the one at the far left is Smn+/−; SMN2+/+, and the other one is Smn+/+; SMN2+/+), the two mice in the middle are type III SMA mice (Smn−/−; SMN2+/+) that were treated as embryos with 20 μg of ASO 10–27, and the two SMA mice (Smn−/−; SMN2+/+) on the right received no treatment. The enlargements show the right ears of one treated and one untreated mouse.