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. Author manuscript; available in PMC: 2010 Jul 30.
Published in final edited form as: Opt Lett. 2010 Jan 1;35(1):1–3. doi: 10.1364/OL.35.000001

Table 1.

Experimental parameters.

λ (nm) PRF (Hz) t (s) TBscan (s) Tmax (s) α (mrad)
570 and 578* 600** 7×10−9 2.7** 7200** 200***

λ: wavelength; PRF: pulse repetition frequency; t: duration of a single pulse; TBscan: exposure duration of each B-scan; Tmax: duration of a complete exposure; α: angular subtense.

*

Because 570 and 578 nm are close to each other and show minimal differences in terms of the safety limits, we use 570 nm throughout the calculation.

**

In the experiments shown in Figs. 2a and 3, the imaged region of interest is 4 mm by 4 mm, with a scanning step size of 2.5 μm. Unidirectional cross-sectional scanning (B-scan) is utilized to avoid the effect due to the backlash of the mechanical scanner. Each forward B-scan takes ~2.7 s, and the scanner reposition takes another ~1.8 s. The total image acquisition time is ~2 hr (7200 s), which is limited by the mechanical scanning speed.

***

The angular subtense is estimated by the numerical aperture of the microscope objective, which is 0.1 in our current system.