Table 1.
λ: wavelength; PRF: pulse repetition frequency; t: duration of a single pulse; TBscan: exposure duration of each B-scan; Tmax: duration of a complete exposure; α: angular subtense.
Because 570 and 578 nm are close to each other and show minimal differences in terms of the safety limits, we use 570 nm throughout the calculation.
In the experiments shown in Figs. 2a and 3, the imaged region of interest is 4 mm by 4 mm, with a scanning step size of 2.5 μm. Unidirectional cross-sectional scanning (B-scan) is utilized to avoid the effect due to the backlash of the mechanical scanner. Each forward B-scan takes ~2.7 s, and the scanner reposition takes another ~1.8 s. The total image acquisition time is ~2 hr (7200 s), which is limited by the mechanical scanning speed.
The angular subtense is estimated by the numerical aperture of the microscope objective, which is 0.1 in our current system.