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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Immunol Rev. 2010 Jan;233(1):181–202. doi: 10.1111/j.0105-2896.2009.00865.x

Fig. 6. Effects of ER stress on IL-12/23 subunit mRNA induction by LPS.

Fig. 6

BM-derived macrophages from B6 mice were untreated or pre-treated with varying concentrations of thapsigargin (Tpg) (0 – 1 μM) for 1 hr, followed by LPS (10 ng/ml) for an additional 2 h. RNA was isolated and mRNA quantified with qPCR. All values have been normalized to GAPDH mRNA. Each data point is the mean of triplicate cultures with standard deviation shown. Effects of LPS alone are shown at ‘0’ Tpg. Asterisks indicate P < 0.05 compared to respective controls without LPS.