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. 2010 Jul 29;6(7):e1001025. doi: 10.1371/journal.ppat.1001025

Figure 3. HilC and RtsA amplify SPI1 gene expression.

Figure 3

(A) Comparison of time-course dynamics for PhilD (pSS074, black) and PhilA (pSS077, red) promoter activities in wild type (solid lines) and a ΔhilC ΔrtsA mutant (CR350, dashed lines) as determined using luciferase transcriptional reporters. (B and C) Comparison of PhilA (pSS055, B) and PhilD (pSS072, C) promoter activities in wild type (black) and a ΔhilC ΔrtsA mutant (CR350, grey) as determined using GFP transcriptional reporters and flow cytometry. Note that the loss of HilC and RtsA causes both a delay and decrease in PhilA promoter activity whereas it causes only a decrease in activity in the case of the PhilD promoter. Experiments were performed as described in Figure 1 .