FIG 1 .

Time course of temperature shifts used to generate E. coli cultures for ChIP-chip analysis. The E. coli strain CMT940 used for these experiments carries the dnaC2 allele and can initiate chromosome replication normally at 30°C but not at 42°C. (A) Rates of DNA synthesis measured by thymidine incorporation (solid line) at different temperatures (dotted line) and the time points (A, B, and C) at which cultures were harvested for analysis. (B) Results from flow cytometry experiments with CMT940 cells at each time point.