FIG 3 .

Relationship between SeqA binding and the occurrence of GATC motifs in vivo. The SeqA binding values are Cy5/Cy3 ratios generated from ChIP experiments analyzed using a DNA microarray. DNA microarray probes were grouped according to the number of GATC motifs present in the probe, and the average Cy5/Cy3 ratio was calculated for each group of probes. Data presented were generated from unsynchronized cultures of E. coli (time point A), cultures where chromosome replication had been blocked for 1 h (time point B), and cultures where chromosome replication had been reinitiated in synchronicity for a period of 6 min (time point C).