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. 2010 May 18;1(1):e00057-10. doi: 10.1128/mBio.00057-10

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Copyright © 2010 Gilchrist et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License, which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited.

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FIG 1 — Measurement of amebic morphology. The Amnis Imagestream imaging cytometer was used to measure the morphology of fixed amebic trophozoites. (A) Bright-field image of an elongated trophozoite. (B) The pixels which constituted the bright-field image of the trophozoites (MASK). From the mask, the computational features of area and circular morphology were derived. A high circularity score resulted from an internally consistent measurement of cell radius. Representative images show trophozoites characterized and “tagged” as either circular (C) or elongated (D).