FIG 5 .
A kinD deletion restores sporulation to the eps tasA matrix mutant under biofilm-inducing conditions. (A) Flow cytometry of cells harboring the PsspB-yfp promoter fusion. Cells were grown on MSgg solid medium at 30°C for 72 h prior to harvesting and disruption of the biofilm for flow cytometry. The control is wild-type cells with no fluorescent protein. AU, arbitrary units. (B) Sporulation of wild type, eps tasA, kinD, and kinD eps tasA cells. Cells were grown on MSgg solid medium at 30°C for the indicated time prior to harvesting and counting of viable spores after heat treatment. Error bars represent standard errors of the means.