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. 2010 Jul 30;5(7):e11894. doi: 10.1371/journal.pone.0011894

Figure 2. Disulfide bond deletion permit MMP-2560–568 epitope generation by the endogenous pathway in HLA-A*0201+/αvβ3- human tumor cells.

Figure 2

Melanoma cell line M117 and non small cell lung carcinoma line 1355 were transfected with plasmids coding for cystein deleted MMP-2. 48 h later, M134.12 CTL clones were added to tumor cells (E/T ratio 1∶3) and the TNF response was tested after 6 h on wehi-13 cells. Standard deviations were obtained from duplicates. cDNA NA134-A corresponding to the C-terminal part of MMP-2, contains MMP-2560–568 epitope and was used as positive control. Transfection efficiency was controlled with GFP transfected tumor cells. Data are representative of at least two independent experiments. Error bars indicate standard deviations of duplicates. p<0.005 was considered significant.