Figure 4. Mutated MMP-2 proteins are more rapidly degraded than the wild-type enzyme.
COS-7 cells transfected with indicated plasmids were pulse-labeled with [35S] methionine/cystein for 15 min and chased for 0–24 h. MMP-2 immunoprecipitates were separated by SDS-PAGE and analyzed by autoradiography (A). Data were plotted to indicate the residual protein remaining where the amount of this protein at 0 h time point was calculated to represent 100% of total MMP-2 in each case (B). Data are representative of at least two independent experiments.