Table 5.

Specificity of TaqMan PCR for detection of oprL and the AES-I HW2 locus

		TaqMan assay
Samplea	No of samples tested	oprL	HW2
		Ct TAMRAb	Ct FAMb
AES-I sputum	1	31.61	21.33
Non-AES-I sputum	1	28.45	-
AES-I strains	31	23 - 30	18 - 30
Non-AES-I strains	8	28 - 30	-

^a All samples were purified using Whatman FTA Elute cards

^b Fluorescent dye used for primer labeling; see Materials and Methods.