Table 3.
CO2 production by different HS13 transformantsa
| ml CO2/mg yeast (d.w.) ± SD (P value) | ||
|---|---|---|
| Plasmad | 20% Sucrose | 30% Sucrose |
| YEplac195 | 0.401 ± 0.005 | 0.255 ± 0.003 |
| YEpCAF130 | 0.385 ± 0.015 (0.218) | nd |
| YEpCDC10 | 0.425 ± 0.017 (0.140) | nd |
| YEpFUR1 | 0.401 ± 0.009 (0.137) | nd |
| YEpSEC14 | 0.422 ± 0.022 (0.112) | nd |
| YEpYVH1 | 0.383 ± 0.013 (0.095) | nd |
| YEpZUO1 | 0.435 ± 0.019 (0.110) | nd |
| YEpCAF16 | 0.462 ± 0.012 (0.017)* | 0.400 ± 0.030 (0.003)* |
| YEpMFT1 | 0.440 ± 0.030 (0.155) | nd |
| YEpNMT1 | 0.426 ± 0.022 (0.204) | nd |
| YEpORC2 | 0.446 ± 0.018 (0.022)* | 0.402 ± 0.036 (0.012)* |
| YEpSSF2 | 0.385 ± 0.015 (0.175) | nd |
aCells were grown on molasses plates and tested for gas production as described in the Materials and Methods section. Values represent the mean of at least three independent experiments. SD, standard deviation. nd, non-determined. *, P < 0.05 for gas production of the overexpressing strain compared to gas production of the control strain (YEplac195).