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Working with the i3C: (A) Packed chip in a sterile pouch. (B) The cover glass is moved back enabling the cell suspension to be loaded into the chamber. The glass is then closed to cover the chamber and to create a short capillary traction area over the input basin (C), so additional aliquots can be added and replace the cell media. Aliquots can be added until the waste area is full and there is no room for additional liquids to enter (D). See text for specific volumes and procedures.
