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. 2010 Jul 30;5(7):e11897. doi: 10.1371/journal.pone.0011897

Figure 5. Superoxide anion production in cells treated with antioxidant compounds for 24 hours (A) or 1 week (B).

Figure 5

Cultured skin fibroblasts from controls and CoQ10 deficient patients were treated with 5 µM CoQ10, idebenone, CoQ2, or vitamin C. After 24 h (A) or 1 week (B), fibroblasts were collected and superoxide anion generation was assessed by MitoSOX staining and quantified by flow cytometry The Y axis represents the fluorescence intensity of the sample relative to the fluorescence intensity of the control sample (FP/FC), after subtracting the background intensity; the X axis represent the sample. 20,000 cells were analyzed in every experiment. Results are expressed as mean ± SD of three experiments. P1 = COQ9 mutant; P2 =  COQ2 mutant; P3 =  COQ2 mutant; P4 = PDSS2 mutant. ***P<0.001 compared with untreated control; ##P<0.01 and ###P<0.001 compared with untreated P1; +++P<0.001 compared with untreated P2; φφ P<0.01 and φφφ P<0.001 compared with untreated P3.