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. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: Cancer Res. 2010 Jul 7;70(15):6377–6383. doi: 10.1158/0008-5472.CAN-10-1180

Figure 2. TMEPAI knockdown blocks TGF-β stimulated growth, migration and invasion.

Figure 2

A. Lack of TMEPAI protein expression in MDA-MB-231 cells stably expressing 2 different TMEPAI shRNAs. B. Growth curves of wild type MDA-MB-231 cells and cells expressing control shRNA and TMEPAI shRNA 1 and 2 with or without TGF-β. Cell proliferation was measured by quantitating total DNA. C. Time course of TMEPAI expression in MDA-MB-231 cells with exposure to TGF-β. Asterisk (*) indicates non-specific antibody reactive band. D. Effect of TGF-β and TMEPAI shRNA on relative invasion of MDA-MB-231 breast cancer cells in Transwell Matrigel invasion assay. Invasion index measured as relative migration of cells across the Matrigelin all groups was calculated.