Skip to main content
. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: Methods. 2010 Feb 1;51(4):444–451. doi: 10.1016/j.ymeth.2010.01.033

Table 2.

Conditions for Primers used in QPCR assay

genome amplicon
size		 cycles Temp
(°C)		 Primer
concentrationcon
(μm, each)		 MgO(Ac)2
centration
(μm)
C. elegans
nuclear 13.7 kb 21 68 0.4 1.2
nuclear 9.3 kb 20 68 0.4 1.2
nuclear 225 bp 23 63 0.4 1.2
mitochondrial 10.9 kb 16 66 0.4 1.2
mitochondrial 195 bp 18 63 0.3 1.2
D. melanogaster
nuclear 11.5 kb 22 67 0.4 1.2
nuclear 10.3 kb 22 67 0.4 1.2
nuclear 152 bp 24 65 0.4 1.2
mitochondrial 14.2 kb 17 66 0.4 1.2
mitochondrial 151 bp 19 61 0.4 1.2
Adenovirus type 2
1 kb 18-19 65 0.4 1.2
F. heteroclitus (Atlantic killish)
nuclear 11.5 kb 24 68 0.4 1.1
nuclear 234 bp 24 62 0.4 1.2
mitochondrial 9.4 kb 16 65 0.4 1.2
mitochondrial 264 bp 24 62 0.4 1.2
D. rerio (zebrafish)
nuclear 10.7 kb 24 69 0.4 1.05
nuclear 233 bp 27 60 0.4 1.2
mitochondrial 10.3 kb 19 68 0.4 1.2
mitochondrial 198 bp 21 62 0.4 1.2