Fig. 3.

Determination of the optical purity of the enantiomers of EMP-CHMC (Panels A & B) and of nPrMP-CHMC (Panels C & D) by use of mutants 3B3 (○-○) and 3D8 (▪-▪). The optical purities of S_P-CMP-CHMC and R_P-iPrMP-CHMC are illustrated in panels E and F, respectively. In all cases, 3D8 was added after termination of CHMC release in presence of 3B3. The latter hydrolyzes exclusively the R_P isomer, whereas 3D8 hydrolyzes both the S_P and R_P enantiomers (Fig. 2B)