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CCN2 expression by primary culture mHSC. (A) RT-PCR of total cellular RNA extracted from primary mHSC for determination of CCN2 gene expression on day 1, day 4 or day 7 of primary culture. A DNA marker ladder and non-cDNA template (NTC) are also shown. (B) Quantitative real-time PCR detection of CCN2 mRNA on days 1–7. Data are the means ± S.D. of triplicate determinations and are representative of three independent experiments. *P < 0.05 versus day 1.
