Table 1.

Chemical and spectral details including source and peak emission wavelength of the different tetracycline derivates in undecalcified bone sections. All fluorochromes were administered subcutaneously at a dose of 30 mg kg⁻¹ bodyweight. Due to the weak fluorescence signal of chlortetracycline and doxycycline, a higher dose of 60 mg kg⁻¹ bodyweight was administered for both derivates. Emission spectrum for minocycline was detectable only in vitro and not in the tissue.

Derivate	Source	Cat.-No.	Molecular formula	Molecular weight	Peak emission wave length [nm]
Chlortetracycline	Fluka	26430	C22H23ClN2O8 HCl	515.34	506
Demeclocycline	Sigma-Aldrich	D6140	C21H21ClN2O8	501.31	535
Doxycycline	Sigma-Aldrich	D9891	C22H24N2O8	512.94	529
Methacycline	Sigma-Aldrich	37906	C22H22N2O8	478.88	541
Minocycline	Sigma-Aldrich	M9511	C23H27N3O7	493.94	(521)
Oxytetracycline	Fluka	75966	C22H24N2O9	496.89	547
Rolitetracycline	Sigma-Aldrich	R2253	C27H33N3O8	527.57	529
Tetracycline	Sigma-Aldrich	T3383	C22H24N2O8	480.90	529