Figure 2.

(A) NF-κB DNA binding activity (EMSA assay) in K562 cells treated either by deferasirox 50 mM for 18 h or by deferioxamine and deferiprone both at 0.5 mM for 30 min. Only after deferasirox incubation is the DNA binding activity of p65 subunit decreased. The first two lanes represent K562 cells incubated with the NF-κB inhibitor PS 1145 as negative control. The black arrow indicates specific binding to the consensus sequence. The graph on the right represents densitometry data. (B) EMSA assay for NF-κB DNA binding activity in K562 cells in basal conditions and after incubation with deferoxamine and deferiprone 50 mM both for 18 h. The DNA binding activity (indicated by the black arrow) is not decreased in both the different conditions of incubation with respect to the one detected in control cells. The graph on the right represents densitometry data.