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. Author manuscript; available in PMC: 2011 Jul 30.
Published in final edited form as: Mol Cell. 2010 Jul 30;39(2):209–221. doi: 10.1016/j.molcel.2010.06.021

Figure 3. Lysylation of EF-P by PoxA in vitro.

Figure 3

[14C]-Lys addition to EF-P catalyzed by PoxA was monitored by SDS-PAGE after various incubation intervals at 37°C. The assay was performed in presence or absence of PoxA and with EF-P wild-type (wt) or the mutant K34A. Proteins were visualized by Coomassie staining (upper panel) and [14C]-Lys-EF-P by phosphorimaging (lower panel). The modification has a mass of 128 Da (Figure S2) and no modification of EF-P by PoxA was observed in the absence of ATP (Figure S3).