Table 2.

Proteins identified as differentially expressed inpoxA mutants.

Gene identifier	Gene name	Fold change (wt/poxA)*	p-value	function
Protein spots identified with higher expression in poxA mutant than in wild-type 14028s background
STM2882	sipA	−2.06	0.02	SPI-1 secreted effector SipA. Binds actin and induces host cytoskeleton rearrangements to promote membrane ruffling and bacterial internalization.
STM2898	invG	− 2.51	0.0087	Outer membrane secretin precursor InvG. Forms ring-shaped structure in outer membrane as part of TTSS.
STM2884	sipC	− 5.71	0.0066	SPI-1 secreted translocase SipC. Translocates secreted proteins into host cells.
STM2874	prgH	− 1.63	0.022	Needle complex inner membrane protein PrgH. Forms multimeric ring-shaped structures with PrgK as part of TTSS.
STM2883	sipD	− 2.08	0.0095	SPI-1 secreted translocase SipD. Translocates secreted proteins into host cells.
STM0830	glnH	− 6.02	0.031	Glutamine ABC transporter periplasmic protein
STM0013	dnaJ	−2.57	0.018	Chaperone protein DnaJ. Prevents aggregation of stress-denatured proteins in response to hyperosmotic and heat shock
STM1130	yjhT	− 10.9, −6.07	0.013	Kelch-domain containing protein
STM2370	pdxB	−1.67	0.04	Erythronate-4-phosphate dehydrogenase PdxB. Catalyzes the formation of 3-hydroxy-4-phospho-hydroxy-alpha-ketobutyrate from erythronate-4-phosphate.
STM0413	Tsx	− 2.35	0.029	Nucleoside channel Tsx. Receptor of phage T6 and colicin K.
STM2886	sicA	− 3.14	0.0087	SPI-1 secretion chaperone SicA. Partitioning factor for SipB and SipC to prevent their premature association.
STM1091	sopB	− 6.79	0.0066	SPI-1 secreted effector SopB. Hydrolyzes inositol phosphates and phosphoinositides.
STM1451	Gst	−2.97	0.0094	Glutathione S-transferase. Catalyzes the conjugation of glutathione with a wide range of endogenous and xenobiotic alkylating agents.
STM0608	ahpC	−1.88	0.0051	Alkyl hydroperoxide reductase subunit AhpC. Catalyzes the conversion of alkyl hydroperoxides to their corresponding alcohols.
STM1133	STM1133	−2.48	0.013	Putative dehydrogenase

Protein spots identified with higher expression in wild-type than in poxA mutant
STM4007	glnA	1.68	0.0091	Glutamine synthetase GlnA. Forms glutamine from ammonia and glutamate with the conversion of ATP to ADP.
STM1830	manX	4.89	0.011	Mannose-specific enzyme IIAB. Imports hexoses (including mannose, glucose, glucosamine, and fructose), releasing the phosphate esters into the cell cytoplasm in preparation for metabolism, primarily via glycolysis.
STM4581	yjjK	4.58	0.0032	Putative ABC transporter ATP-binding protein
STM0215	Map	2.6	0.0089	Methionine aminopeptidase Map. Catalyzes the removal of N-terminal amino acids from peptides and arylamides.
STM3996	yihE	2	0.046	Serine/threonine protein kinase YihE. Catalyzes the phosphorylation of protein substrates at serine and threonine residues.
STM3865	atpD	3.81	0.013	ATP synthase beta subunit AtpD. Produces ATP from ADP in the presence of a proton gradient across the membrane. The beta chain is a regulatory subunit.
STM0831	Dps	1.5	0.015	DNA starvation/stationary phase protection protein Dps. Binds DNA nonspecifically to protect DNA from damage.
STM4149	rplK	1.63	0.014	50S ribosomal protein L11. Binds directly to 23S ribosomal RNA.

^*Proteins with negative fold changes are more highly expressed in the poxA mutant than in the wild-type strain.