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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: Pain. 2010 Apr 18;149(3):522–528. doi: 10.1016/j.pain.2010.03.024

Figure 2.

Figure 2

RTX-treated and untreated corneas were histologically indistinguishable using H&E and PAS staining. Corneas were acquired 24 hr after treatment with 2 µg RTX, post-fixed in 10% formalin, and embedded in methyl methacrylate. Sections were stained with H&E or PAS and evaluated by a masked observer. “E” indicates the epithelial layer and “S” indicates the stromal layer. (A) H&E untreated; (B) PAS untreated; (C) H&E 2 µg RTX; (D) PAS 2 µg RTX. Scale bar = 200 µm. (E) RT-PCR shows TRPV1 transcripts are expressed in trigeminal ganglia (TG) but not the cornea, using GPDH as a normalizing control. Thus, RTX likely has no direct effect on resident corneal cells.