Corneal wound healing rates were not significantly different for eyes treated with 20 µg RTX or vehicle (A). The epithelial layer was removed from an area of the cornea by applying a circle of filter paper soaked in n-heptanol to the cornea for 30 s, followed by copious saline flushing. The wound was visualized at 0, 10, 15, 22, 32, 37, and 42 hr by applying fluorescein solution to the cornea under UV light and the width of the wound was measured with digital calipers in the dorsal-ventral and medial-lateral dimensions. 2-way ANOVA identified no significant difference between average wound widths of RTX- and vehicle-treated eyes at any time point. The area of debridement contracted over time as it was reepithelialized from the edge, and complete reepithelialization occurred by 42 hours in all eyes (B, C, and D). Scale bar: 2 mm.