Fig. 3.

Substrate specificity of mosquito GDC and ADC. Reaction mixtures (1.0 ml each) containing 50 μg of AnGDC or AeADC and 5 mM glutamate or aspartate were incubated at 25 °C. At 10 min after incubation, the reaction mixture was mixed with equal volume of OPT derivatizing agent and then analyzed by HPLC with electrochemical detection. Chromatogram A shows the retention time of β-alanine (4.9 min) and GABA (6.8 min) standard under the applied HPLC conditions. Chromatograms B & C illustrate the relative amounts of GABA and β-alanine, produced in the AnGDC reaction mixture containing glutamate and aspartate respectively. Chromatograms D & E shows the relative amounts of β-alanine and GABA, produced in AeADC reaction mixtures containing aspartate and glutamate respectively.