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. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: J Immunol. 2008 Aug 1;181(3):1927–1936. doi: 10.4049/jimmunol.181.3.1927

Figure 5. Down-regulation of SLP-2 prevents sustained T cell signalling.

Figure 5

A) Jurkat T cells were nucleofected with two different sets of siRNA for SLP-2 or control siRNA and used for stimulation with APC and SEE for the indicated times. Cell lysates were prepared and immunoblotted for dually phosphorylated, active ERK-1/-2 (pERKs), total ERK-1/-2 (tERKs), phospho-PLC-γ1, total PLC-γ1, and SLP-2. B) Signals for activated ERK-1/-2 and total ERK were quantified for each group at the indicated time points for three independent experiments and plotted as normalized densitometric units (mean ± s.d.) for active ERK-1/-2. *: p<0.05, **: p<0.01.