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. Author manuscript; available in PMC: 2011 Oct 1.
Published in final edited form as: Protein Expr Purif. 2010 Jun 8;73(2):152–160. doi: 10.1016/j.pep.2010.06.001

Fig 2. Expression screen in insect cells using three baculovirus vectors.

Fig 2

A. Three baculovirus vectors were used for the infection of Sf9 cells: FB-VMAT2 (lane 1), FBG-VMAT2 (lane 2) and FBU-VMAT2 (lane 3). Sf9 cells were harvested 72 hrs post infection, washed in lysis buffer and sonicated. Samples of the lysates (equal to original volume of 10 μl infected cells in suspension) were separated by SDS-PAGE and Western blot analysis with anti-HA antibody was performed. B. Sf9 cells infected using the FB-VMAT2 vector were grown without or with the addition of glucose, Asn, Gln and FBS after the first 24 hours of infection. Cells were harvested 2,3,4 or 5 days (as indicated) post infection and cell lysates were prepared and assayed for [3H]TBZOH binding. The amount of VMAT2 was calculated from the TBZ binding results.