Table 1.
Cell-free synthesis reactions were carried out in the presence of various detergents.
Detergent present during synthesis | concentartion | Expression |
---|---|---|
CHAPS | 8mM | + |
DDM | 0.02% | + |
Triton ×100 | 0.021% | + |
Cholate | 0.6% | − |
Anzergent 3–14 | 0.2mM | + |
LDAO | 2mM | + |
C12E8 | 0.11mM | + |
Fos-Choline 14 | 0.12mM | + |
Brij 35 | 0.1% | + |
Brij 58 | 0.15% | + |
Brij 78 | 0.1% | + |
Brij 98 | 0.1% | + |