Increased positional errors of the Bcd gradient in nej embryos. (A) δB, standard deviation of measured Bcd fluorescent intensities in the early subgroup of wt (red circles) and nej (blue squares) embryos (note that the mean values of δB are equal to the size of the error bars shown in Fig. 1. Error bars are from bootstrapping (44)). Solid lines are corresponding fits from our theory, outlined in the text. (B) Same as panel A, except for the late subgroup. (C) Measured positional error WBcd/L (for the standard deviation of measured threshold positions, see text) for Bcd gradients in the early subgroup of wt (red circles) or nej (blue squares) embryos as a function of x/L. Error bars are from bootstrapping. Solid lines are corresponding theoretical fits. (D) Same as panel C, except for the late subgroup. (E) Ratio of slopes of the early Bcd profiles for nej and wt as function of position in the embryo. (F) As in panel E, but for late data sets.