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. 2010 Aug 4;99(3):853–861. doi: 10.1016/j.bpj.2010.05.027

Figure 1.

Figure 1

Schematic of the FRET assay used to measure integrin microclustering with donor and acceptor FRET reporters. Energy transfer is measured (top) before the plasma membrane cholesterol level is altered, (middle) after the concentration of cholesterol is reduced, and (bottom) after cholesterol levels are restored to approximately the starting concentration before treatment.