Table 2.

Hot residues determined by PRS analysis

Transition	PDB code	Hot residues∗
GroEL
T→R	1AON (chain H) → 2C7E (chain A)	2, 6, 7, 9, 11, 13, 14, 17, 19, 21, 29, 31, 37, 38, 63, 65, 67, 72, 75, 77, 79, 132, 133, 135, 136, 139, 186, 187, 190–93, 209, 221, 224, 227, 230, 234, 254, 256, 264, 268, 272, 275, 303, 334, 341, 346, 348, 362, 377, 411–414, 417, 418, 453, 455, 456, 459, 461, 464, 467–479, 481–483, 485–493, 506, 508, 518, 521, 523–525
R″→T	1AON (chain A) → 1GR5 (chain A)	5, 6, 9–12, 16–18, 20, 21, 23, 46, 60, 66, 69, 71–73, 90–92, 93, 95, 96, 100, 107, 110, 142, 143, 145, 158, 166, 169, 172, 174, 187, 196, 198, 213, 225, 226, 231, 232, 237, 238, 240–248, 252, 256, 258, 261, 265–268, 271–273, 275, 276, 281, 288, 289, 351–353, 355–359, 362, 363, 367, 375, 380, 387, 402, 450, 522, 525
ADK
Open→closed	4AKE→1AKE	1–3, 6, 9, 11–13, 17–21, 24, 25, 27–48, 50, 51, 57, 63–86, 90, 93–95, 98–104, 108, 110–113, 115–117, 119, 121, 122, 124, 126, 127, 129–132, 139, 142, 145, 146, 148, 151–155, 158, 159, 165, 167, 170, 173–180, 185, 188, 191, 197, 198, 200, 205
Closed→open	1AKE→4AKE	55, 128, 133, 135, 147, 151–155, 167

^∗Residues that yield overlaps >0.7 for GroEL, 0.6 for ADK (open→closed), and 0.5 for ADK (closed→open) in PRS analysis (averaged over 10 runs).