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Thermostability of AKBSUB mutants at Gly-213 and Gly-214 determined by CD
| Protein | Tm (°C) |
|---|---|
| AKBSUB | 48.4 ± 0.2 |
| AKBSUB Q199R | 49.5 ± 1.2 |
| AKBSUB Q199R/G213E | 51.4 ± 0.3 |
| AKBSUB Q199R/G214R | 53.4 ± 0.6 |
| AKBSUB Q199R/G213A | 37.9 ± 0.4 |
| AKBSUB Q199R/G213Q | 39.3 ± 0.9 |
| AKBSUB Q199R/G214A | 49.1 ± 0.1 |
| AKBSUB Q199R/G214K | 48.4 ± 0.6 |
