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Free energy surfaces for the denatured basins of WT and K12M NTL9 at pH 4. Relative free energies (ΔG in kcal/mol) were calculated as a function of radius of gyration Rg (Å) and Cα RMSD (Å) of segment A (top) and segment B (bottom) with respect to the first entry in the NMR structure ensemble (PDB ID: 1CQU).
