Figure 6.

Probability distributions for attractive and repulsive interactions in the denatured states of WT and K12M NTL9 at pH 4. (A) Between Asp⁸ and a basic residue (except for Lys⁷ and Lys¹⁰) in the denatured state of WT. The distributions for Asp⁸-Lys¹² and Asp⁸-Lys¹⁴ are shown in red and green, respectively. (B) Between Lys¹² and an acidic residue in the denatured state of WT. The distribution for Asp⁸-Lys¹² and Glu¹⁷-Lys¹² are shown in red and green, respectively. (C) Between a pair of acidic residues in the denatured state of mutant K12M. The distribution for Asp⁸-Glu⁴⁸ is shown in red. Histograms were made in the range between 0 to 25 Å with a bin width of 0.25 Å.